25: Sam and Mikaela open up the hood to view Bumblebee's engine. Mikaela is impressed with the engine, Sam is impressed with Mikaela. Moments later Mikaela walks away and Sam pursues. Eventually he drives her home and their relationship looks promising. The birth of something new.
45: The Defense Secretary becomes aware of a Decepticon in the desert and wants soldiers who fought it to come home. Moments later a Pentagon analyst realizes she can't break the code; she removes a computer chip to take it to the one hacker who can break the Decepticon's code. Now that I'm aware.
60: Optimus Prime and all other Autobots are landing on Earth. At exactly 60, Sam and Mikaela are looking at a smoldering Autobot that just landed. Moments later Optimus and the other Autobots will gather and inform Sam his grandfather's glasses holds the key to Earth's future. There's no going back now.
-45: The Pentagon experiences a global virus attack caused by the Decepticons. Defense Secretary discovers what happened on Mars, orders ships to turn around, orders all commands to prepare for an imminent attack, orders soldiers and others to Hoover Dam. Discovery.
-25: A Decepticon roller blades through a bus and tackles Optimus, they fight. Optimus wins the fight, indicating who will win the final conflict. The final turning point.
Notice that 45 is usually about escalating the situation and -45 is often about escalating the action to try and resolve the escalating situation.
The next time you look at Transformers, examine 45 and -45: At 45 Pentagon analysts and an expert hacker struggle to understand a code. At -45 the Pentagon experiences a global virus attack caused by the code the analysts couldn't break.
At 45 the Defense Secretary orders soldiers home, at -45 after the virus attack, the Defense Secretary escalates the action: he orders ships to turn around, learns what happened on Mars, and orders soldiers and other protagonists to Hoover dam.